Jumlada 2019-nCoV iyo B.1.617.2 Kala duwanaanta Qalabka Tijaabada Asiidhka Nucleic (PCR-fluorescence probe method) Soo saaraha iyo Warshada |Hecin

2019-nCoV iyo B.1.617.2 Kala duwanaanshaha Qalabka Tijaabada Aashitada Nukliyeerka (habka baaritaanka PCR-fluorescence)

Sharaxaad Gaaban:

Nucleic acid

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Deggan

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Faahfaahinta Alaabta

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Faa'iidooyinka

Tusaalaha: suufka sanka ee bini'aadamka, suufka oropharyngeal, xaakada, shaybaarrada dareeraha dheecaanka alveolar.

Tilmaanta:12×8T/Qalabka;4×24T/Qalabka;

Xaaladaha kaydinta iyo muddada ansaxnimada: Xirmada aan la furin: Ku kaydi inta u dhaxaysa -25℃ ilaa 8℃ meel ka fog iftiinka, shaqaynaysa 18 bilood.

Midabka: FAM (oo loogu talagalay hidda-wadaha N), VIC (loogu talagalay T478K), ROX (L452R), CY5 (heer gudaha)

Astaamaha

N iyo T478K, L452R bartilmaameedka:Saddexda yool ayaa wanaajiya heerka ogaanshaha togan, si weyn u yareeya heerka ogaanshaha la seegay.

Xakamaynta togan waa qayb pseudovirus ah:Jilida qaab dhismeedka cusub ee coronavirus iyo ciyaarista doorka xakamaynta dhabta ah.

Xadka ogaanshaha ugu yar:400 koobi/ml ee 2019-NCOV, 400 koobi/ml ee 2019-NCOV T478K isu beddelka iyo 1000 koobi/ml ee 2019-NCOV L452R beddelka.

Koontaroolo taban iyo kuwa togan ayaa la dejiyay, waxaana la soo bandhigay nidaam caadi ah oo gudaha ah, kaas oo si weyn u yarayn kara faa'iidooyinka been abuurka ah ee uu keeno hawlgalka

Faa'iidooyinka budada la qaboojiyey:fududahay in la qaado, waxqabadka reagent deggan, ma jiraan dhibaatooyin soo noqnoqda ee qaboojinta.

Dejinta qalabka

Tuubooyinka falcelinta/taarikada ayaa la dhex geliyey aaladda waxaana afar kanaal loo doortay FAM, VIC, ROX iyo CY5 muunad kasta.Barnaamijyada gaarka ah Settings waa sida soo socota: