2019-nCoV/IAV/IBV Qalabka Tijaabada Aashitada Nukliyeerka (Qaabka Baaritaanka fluorescence PCR)
Isticmaalka la damacsan yahay
Xirmadani waa tijaabada RT-PCR-waqtiga dhabta ah ee loogu talagalay in lagu ogaado tayada RNA ee 2019-nCoV, Influenza A (IAV), fayraska Hargabka B (IBV).
Faa'iidooyinka
Ogaanshaha iskaashiga:ogaanshaha tayada leh ee RNA ee 2019-nCoV, Fayraska Hargabka A (IAV), fayraska Hargabka B (IBV).
Gaadiidka gudaha 37 ℃ ilaa 3 bilood:reagent lyophilized aad ayuu u deggan yahay;Xaalada gaadiidka: ≤37℃, xasilloon 3 bilood.
Hawl yarXalka falcelinta xoojinta ayaa la isku daray, hoos u dhig hawlgallada shaybaarka.
Noocyada kala duwan ee muunada:Nooca muunada suufka sanka; suufka oropharyngeal; xaakada; shaybaarrada dareeraha dareeraha ee alveolar.
Waxqabad la isku halayn karo:Qiyaasta isku dhaca ee alaabta tixraaca togan ama taban: 100%.isbarbardhigga kala duwanaanshaha qiimaha Ct (CV,%) wuxuu ka yar yahay ama la mid yahay 5%.
Qalabka
UltraFast QPCR FQ-8A, QuantGene 9600 Nidaamka PCR-waqtiga-dhabta ah ；ABI 7500 Nidaamka PCR-waqtiga-dhabta ah,Roche LightCycler96 Nidaamka PCR-waqtiga-dhabta ah,Tianlong TL988-Time PCR System,SLAN-waqtiga dhabta ah PCR System iwm.
Kanaalka fluorescent ayaa loo baahan yahay
Kanaalka ogaanshaha hidda-wadaha: FAM
Kanaalka ogaanshaha IBV: VIC
Kanaalka ogaanshaha IAV: Tex Red
Kanaalka ogaanshaha xakamaynta gudaha: CY5
Xadka Ogaanshaha
2019-nCoV: 400 koobi/ml
Fayraska hargabka A: 1.5 TCID50/mL.
Fayraska hargabka B: 2.0 TCID50/mL.
Gaar ahaan gorfaynta
Qalabkan tijaabada ah waxaa loo isticmaali karaa in lagu ogaado fayraska 2019-nCoV, Hargabka A (2009 H1N1,H1N1,H3N2,H5N1, H7N9), fayraska hargabka B (Yamagata, Victoria) gaar ahaan.
Tilmaamaha alaabta
12X8T
12X4T
HS28-12X8T/Qalabka
HS28-4X24T/Qalabka
