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Fayraska Dengue-ga ah Qalabka Tijaabada Acid Nucleic Acid (habka baaritaanka PCR-fluorescence)
Hordhac
Xirmadan waxaa loogu talagalay in lagu ogaado teeb tayada leh ee fayraska dengue nucleic acid ee serum ama shaybaarrada balaasmaha.Qalabkani wuxuu ku salaysan yahay jajabka gaarka ah ee dhammaan genome ee fayraska dengue nooca 1 ~ 4 si loo naqshadeeyo asal gaar ah iyo TaqMan fluorescent probes nooc kasta, iyo xaqiijinta ogaanshaha degdega ah iyo qorista fayraska dengue iyada oo loo marayo PCR-waqtiga dhabta ah.
Halbeegyada
* Nooca tijaabada ah: Serum ama Plasma * Qalabka codsiga: ABI 7500 Nidaamka PCR-waqtiga-dhabta ah;Bio-rad CFX96;Roche LightCycler480;Nidaamka PCR SLAN. * Kaydinta -25 ℃ ilaa 8 ℃ aan la furin oo ka ilaali iftiinka 18 biloodQaybaha 48T/Qalabka Waxyaabaha ugu muhiimsan DENV-Nooca falcelinta isku dhafka ah, lyophilized 2 tuubbo Furayaasha, shaybaarrada, falcelinta PCR, dNTPs, Enzyme, iwm. DENV xakamaynta togan, lyophilized 1 tube Plasmids for tandem dengue fayraska nooca 1-4 ee ogaanshaha jajabyada bartilmaameedka Xakamaynta xun (Biyaha la nadiifiyey) 1.5ml Biyo sifaysan Buugga isticmaale 1 cutub / Waxqabadka
• Degdeg ah: Waqtiga ugu gaaban ee PCR-ga oo ka mid ah alaabta la midka ah.• Xasaasiyadda sare & takhasuska: Waxay kor u qaadaa ogaanshaha hore ee daaweynta degdega ah.•Awood buuxda oo ka-hortagga faragelinta.Tallaabooyinka hawlgalka
