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Qalabka Tijaabada Aashitada Nukliyeerka ee Ca16 (habka Baaritaanka fluorescence PCR)
Hordhac
CA16 waa cudur-sidaha ugu weyn ee keena cudurka Gacan-afka-foot (HFMD) ee carruurta.Waxaa badanaa la socda Human Enterovirus 71 waxayna ku badan tahay carruurta ka yar 5 sano.Calaamadaha caafimaad ee caabuqa CA16 waa erythema, papules iyo finan yaryar oo ku yaal gacmaha iyo cagaha bukaanka ilmaha, oo ay la socdaan boogaha carrabka iyo xuubka afka.
Qalabkan waxaa loogu talagalay in lagu ogaado tayada tayada ee Coxsackievirus 16 nucleic acid ee serum bini'aadamka ama shaybaarrada balaasmaha.Xirmadani waxay isticmaashaa taxanaha 5'UTR ee aadka loo ilaaliyo ee hidde-sidaha CA16 oo ah gobolka la beegsanayo, wuxuuna naqshadeeyaa asal-raacyo gaar ah iyo baaritaannada fluorescent TaqMan, waxayna xaqiijiyaan ogaanshaha degdegga ah iyo qorista fayraska dengue iyada oo loo marayo PCR-ga-dhabta ah.
Halbeegyada
* Nooca tijaabada: Serum ama Plasma. * Qalabka codsiga: ABI 7500 Nidaamka PCR-waqtiga-dhabta ah;Bio-rad CFX96;Roche LightCycler480;Nidaamka PCR SLAN. * Kaydinta -25 ℃ ilaa 8 ℃ aan la furin oo ka ilaali iftiinka 18 bilood.Qaybaha 48T/Qalabka Waxyaabaha ugu muhiimsan Isku darka falcelinta CA16/IC, lyophilized 2 tuubbo Furayaasha, shaybaarrada, falcelinta PCR, dNTPs, Enzyme, iwm. CA16 xakamayn togan, lyophilized 1 tube Qaybaha pseudoviral oo ay ku jiraan taxanaha bartilmaameedka iyo taxanaha xakamaynta gudaha Xakamaynta xun (Biyaha la nadiifiyey) 3ml Biyo sifaysan xakamaynta gudaha RNA, lyophilized 1 tube Qaybaha Pseudoviral oo ay ku jiraan MS2 Waxqabadka
• Degdeg ah: Waqtiga ugu gaaban ee PCR-ga oo ka mid ah alaabta la midka ah.• Xasaasiyadda sare & takhasuska: Waxay kor u qaadaa ogaanshaha hore ee daaweynta degdega ah.•Awood buuxda oo ka-hortagga faragelinta.• Ogaanshaha noocyo badan oo CA16 ah: Nooca A/Nooca B(B1a,B2&B16)/Nooca C.Tallaabooyinka hawlgalka
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PIV3 Qalabka Tijaabada Ashitada Nucleic (PCR-fluorescence habka baaritaanka)
Hordhac
Fayraska Parainfluenza waa cudur-sidaha neef-mareenka ee muhiimka ah ee dhallaanka iyo carruurta yaryar waana cudurka labaad ee ugu badan ee sambabada iyo boronkiitada ee dhallaanka ka yar 6 bilood.Sababta calaamadaha hargabka: sida qandho, cune xanuun, iwm. Cudurada neef-mareenka hoose sida oof-wareen, boronkiitada, iyo boronkiitada oo sababa caabuq soo noqnoqda, gaar ahaan dhallaanka, waayeelka, iyo dadka difaaca jirkoodu hooseeyo.
Xirmadan waxaa loogu talagalay in lagu ogaado teeb tayada leh ee fayraska Parainfluenza 3 nucleic acid ee serum ama shaybaarrada balaasmaha.Xirmadani waxay isticmaashaa hiddo-wadaha HN ee sida aadka loo ilaaliyo ee hiddo-wadaha PIV3 oo ah gobolka la beegsanayo, wuxuuna naqshadeeyaa asal-raacyo gaar ah iyo baaritaannada fluorescent TaqMan, waxayna xaqiijiyaan ogaanshaha degdegga ah iyo qorista fayraska dengue iyada oo loo marayo PCR-ka-dhabta ah.
Halbeegyada
* Nooca tijaabada: Serum ama Plasma. * Qalabka codsiga: ABI 7500 Nidaamka PCR-waqtiga-dhabta ah;Bio-rad CFX96;Roche LightCycler480;Nidaamka PCR SLAN. * Kaydinta -25 ℃ ilaa 8 ℃ aan la furin oo ka ilaali iftiinka 18 bilood.Qaybaha 48T/Qalabka Waxyaabaha ugu muhiimsan Isku darka falcelinta PIV3/IC, lyophilized 2 tuubbo Furayaasha, shaybaarrada, falcelinta PCR, dNTPs, Enzyme, iwm. PIV3 xakamaynta togan, lyophilized 1 tube Qaybaha pseudoviral oo ay ku jiraan taxanaha bartilmaameedka iyo taxanaha xakamaynta gudaha Xakamaynta xun (Biyaha la nadiifiyey) 3ml Biyo sifaysan xakamaynta gudaha RNA, lyophilized 1 tube Qaybaha Pseudoviral oo ay ku jiraan MS2 IFU 1 cutub Buugga Tilmaamaha Isticmaalaha Waxqabadka
• Degdeg ah: Waqtiga ugu gaaban ee PCR-ga oo ka mid ah alaabta la midka ah.• Xasaasiyadda sare & takhasuska: Waxay kor u qaadaa ogaanshaha hore ee daaweynta degdega ah.•Awood buuxda oo ka-hortagga faragelinta.• Fudud: Looma baahna goobo dheeraad ah oo ka-hortagga faddaraynta.Tallaabooyinka hawlgalka
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Qalabka Tijaabada Ashitada Nucleic Acid (RSV)
Hordhac
Calaamadaha ugu muhiimsan ee daaweynta caabuqa fayraska syncytial Neefsashada waa sanka oo xidhma, sinusitis, rajo-la'aan, hiinraag dhicis ah, hawada fadhiista, sanka oo duuban ama ololaya, gashad hoosaad hoose ah iyo xitaa cyanosis.Qandho ma aha calaamadda ugu weyn ee caabuqa RSV, oo kaliya ku dhawaad 50% bukaanada carruurta ayaa leh heerkul dhexdhexaad ah oo kor u kaca, labadaba calaamadaha bronchiolitis iyo oof-wareen ayaa laga yaabaa inay soo baxaan isku mar.Infekshanka RSV ee dadka qaangaarka ah ayaa leh astaamo fudud ama ma laha, laakiin wuxuu u keeni karaa infekshanno aad u daran dadka waayeelka ah ama bukaannada difaacooda liita.
Xirmadan waxaa loogu talagalay in lagu ogaado teeb tayada leh ee fayraska dengue nucleic acid ee serum ama shaybaarrada balaasmaha.Xirmadani waxay isticmaashaa taxanaha N hiddo-wadaha aadka loo xafiday ee hiddo-wadaha RSV sida gobolka la beegsanayo, oo nashqadeeya aasaasiyaal gaar ah iyo TaqMan fluorescent probes, oo xaqiijisa ogaanshaha degdega ah iyo qorista fayraska dengue iyada oo loo marayo PCR-da-dhabta ah.
Halbeegyada
* Nooca tijaabada: Serum ama Plasma. * Qalabka codsiga: ABI 7500 Nidaamka PCR-waqtiga-dhabta ah;Bio-rad CFX96;Roche LightCycler480;Nidaamka PCR SLAN. * Kaydinta -25 ℃ ilaa 8 ℃ aan la furin oo ka ilaali iftiinka 18 bilood.Qaybaha 48T/Qalabka Waxyaabaha ugu muhiimsan Isku darka falcelinta RSV/IC, lyophilized 2 tuubbo Furayaasha, shaybaarrada, falcelinta PCR, dNTPs, Enzyme, iwm. xakamaynta togan ee RSV, lyophilized 1 tube Qaybaha pseudoviral oo ay ku jiraan taxanaha bartilmaameedka iyo taxanaha xakamaynta gudaha Xakamaynta xun (Biyaha la nadiifiyey) 3ml Biyo sifaysan xakamaynta gudaha RNA, lyophilized 1 tube Qaybaha Pseudoviral oo ay ku jiraan MS2 IFU 1 cutub Buugga Tilmaamaha Isticmaalaha Waxqabadka
• Degdeg ah: Waqtiga ugu gaaban ee PCR-ga oo ka mid ah alaabta la midka ah.• Xasaasiyadda sare & takhasuska: Waxay kor u qaadaa ogaanshaha hore ee daaweynta degdega ah.•Awood buuxda oo ka-hortagga faragelinta.• Ogaanshaha noocyo badan oo RSV ah: Serotypes A&B.Tallaabooyinka hawlgalka
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EV71 Qalabka Tijaabada Aashitada Nukliyeerka (PCR-Fluorescence Habka)
Hordhac
Calaamadaha caafimaad ee ugu muhiimsan ee caabuqa EV71 waa: bukaanada qaba, gaar ahaan carruurta, maqaarka iyo xuubka xuubka iyo boogaha gacmaha, cagaha, afka iyo qaybaha kale, badankooduna waxaa weheliya calaamado nidaamsan sida qandho, anorexia, daal, iyo caajisnimo.Caabuqyada fudud waxay keeni karaan shuban, qandho, finan herpetic ah, meningitis aseptic, encephalitis, myocarditis, iyo xaalado daran waxay u muuqan karaan sida curyaannimo degdeg ah (AFP), barar sambabada ama dhiig-baxa, iyo xitaa dhimasho.EV71 waxay inta badan ku dhacdaa dhallaanka iyo carruurta yaryar, gaar ahaan carruurta ka yar 5 sano.
Qalabkan waxaa loogu talagalay in lagu ogaado tayada teebaynta ee Human Enterovirus 71 nucleic acid ee serum ama shaybaarrada balaasmaha.Xirmadani waxay isticmaashaa taxanaha 5'UTR ee aadka loo ilaaliyo ee hiddo-wadaha EV71 oo ah gobolka la beegsanayo, waxayna naqshadaysaa asal-raacyo gaar ah iyo baaritaannada fluorescent TaqMan, waxayna xaqiijisaa ogaanshaha degdegga ah iyo qorista fayraska dengue iyada oo loo marayo PCR-ga-dhabta ah.
Halbeegyada
* Nooca tijaabada: Serum ama Plasma. * Qalabka codsiga: ABI 7500 Nidaamka PCR-waqtiga-dhabta ah;Bio-rad CFX96;Roche LightCycler480;Nidaamka PCR SLAN. * Kaydinta -25 ℃ ilaa 8 ℃ aan la furin oo ka ilaali iftiinka 18 bilood.Qaybaha 48T/Qalabka Waxyaabaha ugu muhiimsan Isku darka falcelinta EV71/IC, lyophilized 2 tuubbo Furayaasha, shaybaarrada, falcelinta PCR, dNTPs, Enzyme, iwm. EV71 xakamaynta togan, lyophilized 1 tube Qaybaha pseudoviral oo ay ku jiraan taxanaha bartilmaameedka iyo taxanaha xakamaynta gudaha Xakamaynta xun (Biyaha la nadiifiyey) 3ml Biyo sifaysan xakamaynta gudaha RNA, lyophilized 1 tube Qaybaha Pseudoviral oo ay ku jiraan MS2 IFU 1 cutub Buugga Tilmaamaha Isticmaalaha Waxqabadka
• Degdeg ah: Waqtiga ugu gaaban ee PCR-ga oo ka mid ah alaabta la midka ah.• Xasaasiyadda sare & takhasuska: Waxay kor u qaadaa ogaanshaha hore ee daaweynta degdega ah.•Awood buuxda oo ka-hortagga faragelinta.• Helitaanka noocyo badan oo genotypes ah oo ah EV71: A, B1,B3,C1,C2,C3,C4&C5.Tallaabooyinka hawlgalka
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Qalabka Tijaabada Aashitada Nucleic-ka ah ee EV (habka baaritaanka fluorescence PCR)
Hordhac
Calaamadaha caafimaad ee ugu waaweyn ee caabuqa EV waa: bukaanada qaba, gaar ahaan carruurta, maqaarka iyo xuubka xuubka herpes iyo boogaha gacmaha, cagaha, afka iyo qaybaha kale, badankoodana waxaa la socda calaamado nidaamsan sida qandho, anorexia, daal, iyo caajisnimo.Caabuqyada fudud waxay keeni karaan shuban, qandho, finan herpetic ah, meningitis aseptic, encephalitis, myocarditis, iyo xaalado daran waxay u muuqan karaan sida curyaannimo degdeg ah (AFP), barar sambabada ama dhiig-baxa, iyo xitaa dhimasho.EV waxay inta badan ku dhacdaa dhallaanka iyo carruurta yaryar, badi carruurta da'doodu ka yar tahay 5 sano.
Xirmadan waxaa loogu talagalay in lagu ogaado tayada wax lagu qoro ee enterovirus nucleic acid ee serum ama shaybaarrada balaasmaha.Xirmadani waxay isticmaashaa taxanaha 5'UTR ee aadka loo ilaaliyo ee hiddo-wadaha EV oo ah gobolka la beegsanayo, waxayna naqshadaysaa asal-raacyo gaar ah iyo baaritaannada fluorescent TaqMan, waxayna xaqiijisaa ogaanshaha degdegga ah iyo qorista fayraska dengue iyada oo loo marayo PCR-ga-dhabta ah.
Halbeegyada
* Nooca tijaabada: Serum ama Plasma. * Qalabka codsiga: ABI 7500 Nidaamka PCR-waqtiga-dhabta ah;Bio-rad CFX96;Roche LightCycler480;Nidaamka PCR SLAN. * Kaydinta -25 ℃ ilaa 8 ℃ aan la furin oo ka ilaali iftiinka 18 bilood.Qaybaha 48T/Qalabka Waxyaabaha ugu muhiimsan Isku darka falcelinta EV/IC, lyophilized 2 tuubbo Furayaasha, shaybaarrada, falcelinta PCR, dNTPs, Enzyme, iwm. Xakamaynta togan ee EV, lyophilized 1 tube Qaybaha pseudoviral oo ay ku jiraan taxanaha bartilmaameedka iyo taxanaha xakamaynta gudaha Xakamaynta xun (Biyaha la nadiifiyey) 3ml Biyo sifaysan xakamaynta gudaha RNA, lyophilized 1 tube Qaybaha Pseudoviral oo ay ku jiraan MS2 IFU 1 cutub Buugga Tilmaamaha Isticmaalaha Waxqabadka
• Degdeg ah: Waqtiga ugu gaaban ee PCR-ga oo ka mid ah alaabta la midka ah.• Xasaasiyadda sare & takhasuska: Waxay kor u qaadaa ogaanshaha hore ee daaweynta degdega ah.•Awood buuxda oo ka-hortagga faragelinta.• Ogaanshaha noocyo badan oo Human EV: CA, CB, EV71&Echovirus.Tallaabooyinka hawlgalka
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PIV1 Qalabka Tijaabada Ashitada Nucleic (PCR-fluorescence habka baaritaanka)
PIV1 Qalabka Tijaabada Ashitada Nucleic (PCR-fluorescence habka baaritaanka)
Hordhac
Fayraska Parainfluenza waa cudur-sidaha neef-mareenka ee muhiimka ah ee dhallaanka iyo carruurta yaryar waana cudurka labaad ee ugu badan ee sambabada iyo boronkiitada ee dhallaanka ka yar 6 bilood.Sababta calaamadaha hargabka: sida qandho, cune xanuun, iwm. Cudurada neef-mareenka hoose sida oof-wareen, boronkiitada, iyo boronkiitada oo sababa caabuq soo noqnoqda, gaar ahaan dhallaanka, waayeelka, iyo dadka difaaca jirkoodu hooseeyo.
Xirmadan waxaa loogu talagalay in lagu ogaado teeb tayada leh ee fayraska Parainfluenza 1 nucleic acid ee serum bini'aadamka ama shaybaarrada balaasmaha.Xirmadani waxay isticmaashaa hiddo-wadaha HN ee sida aadka ah loo ilaaliyo ee hiddo-wadaha PIV1 oo ah gobolka la beegsanayo, wuxuuna naqshadeeyaa asal-raacyo gaar ah iyo baaritaannada fluorescent TaqMan, waxayna xaqiijiyaan ogaanshaha degdegga ah iyo qorista fayraska dengue iyada oo loo marayo PCR-da-waqtiga-dhabta ah.
Halbeegyada
* Nooca tijaabada: Serum ama Plasma. * Qalabka codsiga: ABI 7500 Nidaamka PCR-waqtiga-dhabta ah;Bio-rad CFX96;Roche LightCycler480;Nidaamka PCR SLAN. * Kaydinta -25 ℃ ilaa 8 ℃ aan la furin oo ka ilaali iftiinka 18 bilood.Qaybaha 48T/Qalabka Waxyaabaha ugu muhiimsan Isku darka falcelinta PIV1/IC, lyophilized 2 tuubbo Furayaasha, shaybaarrada, falcelinta PCR, dNTPs, Enzyme, iwm. PIV1 xakamaynta togan, lyophilized 1 tube Qaybaha pseudoviral oo ay ku jiraan taxanaha bartilmaameedka iyo taxanaha xakamaynta gudaha Xakamaynta xun (Biyaha la nadiifiyey) 3ml Biyo sifaysan xakamaynta gudaha RNA, lyophilized 1 tube Qaybaha Pseudoviral oo ay ku jiraan MS2 IFU 1 cutub Buugga Tilmaamaha Isticmaalaha Waxqabadka
• Degdeg ah: Waqtiga ugu gaaban ee PCR-ga oo ka mid ah alaabta la midka ah.• Xasaasiyadda sare & takhasuska: Waxay kor u qaadaa ogaanshaha hore ee daaweynta degdega ah.•Awood buuxda oo ka-hortagga faragelinta.• Fudud: Looma baahna goobo dheeraad ah oo ka-hortagga faddaraynta.Tallaabooyinka hawlgalka
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IAV/IBV/ADV Qalabka Tijaabada Aashitada Nucleic (PCR-Fluorescence baarista)
Hordhac
Fayraska hargabka A, hargabka B iyo Human adenovirus waxay dhamaantood keeni karaan caabuqyada neef-mareenka oo leh calaamado caafimaad oo isku mid ah, gaar ahaan qandho, qufac, sanka oo istaaga, cunaha oo raaxo leh, daal, madax xanuun, murqo xanuun iyo calaamado kale, bukaanada qaarna waxaa weheliya gabaabsi neefta, boronkiitada ama oof-wareen.
Xirmadan waxaa loogu talagalay in lagu ogaado teeb tayada leh ee Fayraska Hargabka A (IAV), fayraska Hargabka B (IBV) iyo Aden adenovirus (ADV) nucleic acid ee ku jira shaybaarrada serum ama balaasmaha.Xirmadani waxay isticmaashaa hiddo-wadaha sida aadka ah loo ilaaliyo ee IAV, IBV iyo ADV hidda sida gobolka la beegsanayo, oo nashqadeeya furayaal gaar ah iyo TaqMan fluorescent probes, oo xaqiijisa ogaanshaha degdega ah iyo ku qorista fayraska dengue iyada oo loo marayo PCR-waqtiga dhabta ah.
Halbeegyada
* Nooca tijaabada: Serum ama Plasma. * Qalabka codsiga: ABI 7500 Nidaamka PCR-waqtiga-dhabta ah;Bio-rad CFX96;Roche LightCycler480;Nidaamka PCR SLAN. * Kaydinta -25 ℃ ilaa 8 ℃ aan la furin oo ka ilaali iftiinka 18 bilood.Qaybaha 48T/Qalabka Waxyaabaha ugu muhiimsan Isku darka falcelinta IAV/IBV/ADV/IC, lyophilized 2 tuubbo Furayaasha, shaybaarrada, falcelinta PCR, dNTPs, Enzyme, iwm. IAV/IBV/ADV xakamaynta togan, lyophilized 1 tube Qaybaha pseudoviral oo ay ku jiraan taxanaha bartilmaameedka iyo taxanaha xakamaynta gudaha Xakamaynta xun (Biyaha la nadiifiyey) 3ml Biyo sifaysan xakamaynta gudaha RNA, lyophilized 1 tube Qaybaha Pseudoviral oo ay ku jiraan MS2 IFU 1 cutub Buugga Tilmaamaha Isticmaalaha Waxqabadka
• Degdeg ah: Waqtiga ugu gaaban ee PCR-ga oo ka mid ah alaabta la midka ah.• Xasaasiyadda sare & takhasuska: Waxay kor u qaadaa ogaanshaha hore ee daaweynta degdega ah.•Awood buuxda oo ka-hortagga faragelinta.Tallaabooyinka hawlgalka
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Qalabka Tijaabada Ashitada Nucleic Acid (HBoV)
Hordhac
Infekshanka Bocavirus ee bini'aadamka wuxuu inta badan u muuqdaa sida rhinitis, pharyngitis, oof-wareen, otitis media ba'an ama gastroenteritis, iyo calaamadaha sida qufaca, dyspnea, qarqaryo, qandho, lallabbo, matag, iyo shuban ayaa dhici karta.Bocavirus-ka bani-aadmiga waxa uu ku fiican yahay 1% ilaa 10% muunadaha neefsashada ee carruurta iyo dadka waaweyn ee qaba cudurka neef-mareenka ee degdega ah.
Xirmadan waxaa loogu talagalay in lagu ogaado teeb tayada leh ee bocavirus nucleic acid ee serum bini'aadamka ama shaybaarrada balaasmaha.Xirmadani waxay isticmaashaa hiddasidaha VP ee aadka loo ilaaliyo ee hiddo-wadaha HBoV oo ah gobolka la beegsanayo, wuxuuna naqshadeeyaa asal-raacyo gaar ah iyo baaritaannada fluorescent TaqMan, oo xaqiijiya ogaanshaha degdegga ah iyo qorista fayraska dengue iyada oo loo marayo PCR-ga-dhabta ah.
Halbeegyada
* Nooca tijaabada: Serum ama Plasma. * Qalabka codsiga: ABI 7500 Nidaamka PCR-waqtiga-dhabta ah;Bio-rad CFX96;Roche LightCycler480;Nidaamka PCR SLAN. * Kaydinta -25 ℃ ilaa 8 ℃ aan la furin oo ka ilaali iftiinka 18 bilood.Qaybaha 48T/Qalabka Waxyaabaha ugu muhiimsan Isku darka falcelinta HBoV/IC, lyophilized 2 tuubbo Furayaasha, shaybaarrada, falcelinta PCR, dNTPs, Enzyme, iwm. Xakamaynta HBoV togan, lyophilized 1 tube Qaybaha pseudoviral oo ay ku jiraan taxanaha bartilmaameedka iyo taxanaha xakamaynta gudaha Xakamaynta xun (Biyaha la nadiifiyey) 3ml Biyo sifaysan xakamaynta gudaha DNA, lyophilized 1 tube Qaybaha Pseudoviral oo ay ku jiraan M13 IFU 1 cutub Buugga Tilmaamaha Isticmaalaha Waxqabadka
• Degdeg ah: Waqtiga ugu gaaban ee PCR-ga oo ka mid ah alaabta la midka ah.• Xasaasiyadda sare & takhasuska: Waxay kor u qaadaa ogaanshaha hore ee daaweynta degdega ah.•Awood buuxda oo ka-hortagga faragelinta.• Fudud: Looma baahna goobo dheeraad ah oo ka-hortagga faddaraynta.Tallaabooyinka hawlgalka
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Qalabka Tijaabada Aashitada Nukliyeerka ADV (habka baaritaanka florescence PCR)
Hordhac
Adenovirus waa cudur faafa oo muhiim ah oo keena caabuqyada neef-mareenka, iyo noocyada qaarkood xitaa waxay keeni karaan dillaaca caabuqyada neef-mareenka ee degdega ah, gaar ahaan dhallaanka iyo carruurta, oo keena boronkiito daran iyo xitaa oof-wareen, iyo sidoo kale conjunctivitis, encephalitis, cystitis iyo shuban.Waxay ku dhici kartaa dadka da' kasta leh, kooxaha u nugulna waa dhallaanka iyo carruurta da'doodu ka yar tahay 5 sano.
Xirmadan waxaa loogu talagalay in lagu ogaado teeb tayada leh ee adenovirus nucleic acid ee serum bini'aadamka ama shaybaarrada balaasmaha.Xirmadani waxay isticmaashaa hiddasidaha E1A ee taxanaha aadka loo ilaaliyo ee hidde-wadaha ADV oo ah gobolka la beegsanayo, wuxuuna naqshadeeyaa asal-raacyo gaar ah iyo baaritaannada fluorescent TaqMan waxayna xaqiiqsadaan ogaanshaha degdegga ah iyo qorista fayraska dengue iyada oo loo marayo PCR-da-dhabta ah.
Halbeegyada
* Nooca tijaabada: Serum ama Plasma. * Qalabka codsiga: ABI 7500 Nidaamka PCR-waqtiga-dhabta ah;Bio-rad CFX96;Roche LightCycler480;Nidaamka PCR SLAN. * Kaydinta -25 ℃ ilaa 8 ℃ aan la furin oo ka ilaali iftiinka 18 bilood.Qaybaha 48T/Qalabka Waxyaabaha ugu muhiimsan Isku darka falcelinta ADV/IC, lyophilized 2 tuubbo Furayaasha, shaybaarrada, falcelinta PCR, dNTPs, Enzyme, iwm. ADV xakamaynta togan, lyophilized 1 tube Qaybaha pseudoviral oo ay ku jiraan taxanaha bartilmaameedka iyo taxanaha xakamaynta gudaha Xakamaynta xun (Biyaha la nadiifiyey) 3ml Biyo sifaysan xakamaynta gudaha DNA, lyophilized 1 tube Qaybaha Pseudoviral oo ay ku jiraan M13 IFU 1 cutub Buugga Tilmaamaha Isticmaalaha Waxqabadka
• Degdeg ah: Waqtiga ugu gaaban ee PCR-ga oo ka mid ah alaabta la midka ah.• Xasaasiyadda sare & takhasuska: Waxay kor u qaadaa ogaanshaha hore ee daaweynta degdega ah.•Awood buuxda oo ka-hortagga faragelinta.• Ogaanshaha noocyo badan oo ADV ah:
Tallaabooyinka hawlgalka
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Diyaar u ah in la isticmaalo PCR Master Mix
Hordhac
Akhri-si-la Isticmaalo PCR Master Mix waa diyaar in la isticmaalo, lyophilized master mix oo loogu talagalay waxtarka sare ee waqtiga dhabta ah PCR kaas oo loo isticmaali karo ogaanshaha degdega ah ee shaybaarada DNA-da. DNA polymerase, MgCl2 iyo dNTPs.Kaliya dib u samee isku dhafka sare adiga oo ku daraya biyaha heerka PCR oo ay la socdaan qaab-dhismeedkaaga, baaritaanka Taqman iyo furayaasha wadarta mugga 20 µl.
Halbeegyada
* Ku kaydi -25 ℃ ~ 8 ℃.Kaydinta qalalan ee la xidhay, qoyaan la'aan. * Xidhmadan magaciisii hore waa Realtime PCR Master Mix (oo leh dNTPs, lyophilized).CAT maya. Qayb Tilmaamid Tirada Ogow KY133-01 Aad u fudud SYBR Cagaar qPCR Master Mix (oo leh dNTPs, lyophilized) 48T/xirmo 48Tuubooyinka 8-cirif, 0.1ml Biyaha heerka PCR 1.5ml/ Tube 1 Tube Cryotube, 2.0 ml KY133-02 Aad u fudud SYBR Cagaar qPCR Master Mix (oo leh dNTPs, lyophilized) 48T/xirmo 48Tuubooyinka 8-cirif, 0.2ml Biyaha heerka PCR 1.5ml/ Tube 1 Tube Cryotube, 2.0 ml KY133-03 Aad u fudud SYBR Cagaar qPCR Master Mix (oo leh dNTPs, lyophilized) 48T/xirmo 2Tubes Cryotube, 2.0 ml Biyaha heerka PCR 1.5ml/ Tube 1 Tube Cryotube, 2.0 ml KY133-04 Aad u fudud SYBR Cagaar qPCR Master Mix (oo leh dNTPs, lyophilized) 500T/kit 1Tube / Biyaha heerka PCR 10ml 1 Dhalo / Waxqabadka
Isticmaalka ballaaran: Lagu dabaqi karo ogaanshaha badan.• Dareen sare: Wax qabad aad u wanaagsan marka la eego qaab-dhismeedyada hoose.• Ku habboonaanta: Horay loo qasi jiray oo bilaash in la isticmaalo. -
Aad u Fudud SYBR Cagaar RT-qPCR Master Mix
Hordhac
SYBR Cagaaran RT-qPCR Master Mix (oo leh dNTPs, lyophilized) waa diyaar in la isticmaalo, lyophilizedmastermix ee waxtarka sare leh ee RT-qPCR kaas oo loo isticmaali karo ogaanshaha degdega ah ee DNA ama RNAsamples.Themixin waxaa ku jira bilawga kulul ee laba-xiran ee Super HP Taq DNA polymerase, M-MLV transcriptase reverse (RNaseH-), MgCl2, SYBR dheeha cagaaran iyo dNTPs.Kaliya dib u samee isku dhafka sare adiga oo ku daraya biyaha heerka PCR oo ay weheliyaan qaab-dhismeedkaaga, Taqman probes iyo furayaasha wadar ahaan 20 µl.
Halbeegyada
* Ku kaydi -25 ℃ ~ 8 ℃.Kaydinta qalalan ee la xidhay, qoyaan la'aan. * Qalabkan magaciisii hore waa Super-Easy SYBR Cagaar RT-qPCR Master Mix (oo leh dNTP, lyophilized). WaxqabadkaCAT maya. Qayb Tilmaamid Tirada Ogow KY135-01 SYBR Cagaaran RT-qPCR Master Mix (oo leh dNTPs, lyophilized) 48T/xirmo 48Tuubooyinka 8-cirif, 0.1ml Biyaha heerka PCR 1.5mL/Tube 1Tube Cryotube, 2.0 ml KY135-02 SYBR Cagaaran RT-qPCR Master Mix (oo leh dNTPs, lyophilized) 48T/xirmo 48Tuubooyinka 8-cirif, 0.2ml Biyaha heerka PCR 1.5mL/Tube 1Tube Cryotube, 2.0 ml KY135-03 SYBR Cagaaran RT-qPCR Master Mix (oo leh dNTPs, lyophilized) 48T/xirmo 2Tubes Cryotube, 2.0 ml Biyaha heerka PCR 1.5mL/Tube 1Tube Cryotube, 2.0 ml KY135-04 SYBR Cagaaran RT-qPCR Master Mix (oo leh dNTPs, lyophilized) 500T/kit 1Tube / Biyaha heerka PCR 10ml 1 Dhalo / Saxnaanta: Khatarta wasakhda oo yar• Dareen sare: Wax qabad aad u wanaagsan marka la eego qaab-dhismeedyada hoose.• Ku habboonaanta: Horay loo qasi jiray oo bilaash in la isticmaalo. -
Aad u fudud SYBR Cagaar qPCR Master Mix
Hordhac
SYBR Cagaaran qPCR Master Mix (oo leh dNTPs, lyophilized) waa diyaar in la isticmaalo, lyophilized master mixforhigh-efficient realtime PCR amplification kaas oo loo isticmaali karo ogaanshaha degdega ah ee muunado DNA.Bilawga kulul ee Super HP Taq DNA polymerase, PCR Buffer, MgCl2, SYBR dheeha cagaaran iyo dNTPs.Si fudud dib u dhis isku dhafka sayidku adiga oo ku daraya biyaha PCR-fasalka ah oo ay la socdaan templatekaaga iyo furayaashaada wadar ahaan mug dhan 20µlAlaabooyinka RT-qPCR waxay ku habboon yihiin isku xigxiga.
Halbeegyada
* Ku kaydi -25 ℃ ~ 8 ℃.Kaydinta qalalan ee la xidhay, qoyaan la'aan. * Xidhmadan magaciisii hore waa SYBR Cagaaran qPCR Master Mix (oo leh dNTPs, lyophilized)CAT maya. Qayb Tilmaamid Tirada Ogow KY136-01 Aad u fudud SYBR Cagaar qPCR Master Mix (oo leh dNTPs, lyophilized) 48T/xirmo 48Tuubooyinka 8-cirif, 0.1ml Biyaha heerka PCR 1.5ml/ Tube 1 Tube Cryotube, 2.0 ml KY136-02 Aad u fudud SYBR Cagaar qPCR Master Mix (oo leh dNTPs, lyophilized) 48T/xirmo 48Tuubooyinka 8-cirif, 0.2ml Biyaha heerka PCR 1.5ml/ Tube 1 Tube Cryotube, 2.0 ml KY136-03 Aad u fudud SYBR Cagaar qPCR Master Mix (oo leh dNTPs, lyophilized) 48T/xirmo 2Tubes Cryotube, 2.0 ml Biyaha heerka PCR 1.5ml/ Tube 1 Tube Cryotube, 2.0 ml KY136-04 Aad u fudud SYBR Cagaar qPCR Master Mix (oo leh dNTPs, lyophilized) 500T/kit 1Tube / Biyaha heerka PCR 10ml 1 Dhalo / Waxqabadka
Isticmaalka ballaaran: Lagu dabaqi karo ogaanshaha badan.• Dareen sare: Wax qabad aad u wanaagsan marka la eego qaab-dhismeedyada hoose.• Ku habboonaanta: Horay loo qasi jiray oo bilaash in la isticmaalo.
